Targeted heritable mutation and gene conversion by Cas9-CRISPR in Caenorhabditis elegans.
نویسندگان
چکیده
We have achieved targeted heritable genome modification in Caenorhabditis elegans by injecting mRNA of the nuclease Cas9 and Cas9 guide RNAs. This system rapidly creates precise genomic changes, including knockouts and transgene-instructed gene conversion.
منابع مشابه
Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination
Cas9 is an RNA-guided double-stranded DNA nuclease that participates in clustered regularly interspaced short palindromic repeats (CRISPR)-mediated adaptive immunity in prokaryotes. CRISPR-Cas9 has recently been used to generate insertion and deletion mutations in Caenorhabditis elegans, but not to create tailored changes (knock-ins). We show that the CRISPR-CRISPR-associated (Cas) system can b...
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عنوان ژورنال:
- Genetics
دوره 195 3 شماره
صفحات -
تاریخ انتشار 2013